Research Data Package README file

Package Title

Cardinium and Wolbachia co-infection of haplodiploid Pezothrips kellyanus induces cytoplasmic incompatibility, male development and embryonic mortality and post-embryonic mortality.

Package Creation Date

January 6th 2017

Package File Name

Pezothrips_endosymbiotic_coinfection.zip

ID

http://handle.westernsydney.edu.au:8081/1959.7/hiev_144

URL

http://w0297.uws.edu.au/data_files/167679

Description

Data describes multiple crossing experiments involving Pezothrips kellyanus laboratory colonies that are infected with with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). Pezothrips exhibit haplodiploid reproduction and the fecundity and reproductive effects of the endosymbiont complement in various mating combinations, as well as in virgin females, was measured to determine the reproductive outcomes. This data demonstrates the stability of co-infection, the high titre of Wolbachia infection relative to Cardinium in each co-infected individual, and the apparent lack of competition between these bacteria within hosts. Maternal transmission to offspring was complete for Wolbachia and high for Cardinium. Cardinium-induced CI resulted in a combination of male development (MD) and embryonic female mortality of fertilised eggs, and Wolbachia-induced CI resulted in female mortality together with post-embryonic mortality.

Experiment

Pezothrips kellyanus endosymbiotic associations

Facility

Entomology Laboratory Growth Chamber

Added By

Jennifer Morrow

Labels

Cardinium, cytoplasmic incompatibility, embryonic mortality, endosymbiotic bacteria, fecundity, haplodiploid, male development, maternal transmission, Pezothrips, quantitative PCR, Wolbachia

Start time

2013-01-01 12:00:00 +11:00

End time

2017-01-06 11:00:00 +11:00

Access Rights Type

Open

Access Rights String

Dataset is available via the Western Sydney University ResearchDirect record under Attachments section.

Licence

CC BY: Attribution

Licence URI

http://creativecommons.org/licenses/by/4.0

Rights Statement

Copyright University of Western Sydney

Language

English

HDL Handle

Physical Location

Western Sydney University

Grant Numbers

This project was supported by an Australian Postgraduate Award (International) to Duong Nguyen and a research project grant from Western Sydney University to Markus Riegler and Robert Spooner-Hart

Related Websites

http://www.westernsydney.edu.au/hie, http://www.westernsydney.edu.au/hie

Creator

Jennifer Morrow (j.morrow@westernsydney.edu.au)

Contributors

Duong Nguyen (duong.nguyen@westernsydney.edu.au)
Markus Riegler (m.riegler@westernsydney.edu.au)
Robert Spooner-Hart (R.Spooner-Hart@westernsydney.edu.au)

Software Information

Generating Software Application HIEv
Software Version 2.4.02
URLs https://github.com/IntersectAustralia/dc21 www.intersect.org.au
Max Package Size Unlimited

Organisational Information

Research Centre Name Hawkesbury Institute for the Environment
Overarching Entity Western Sydney University
Address Locked Bag 1797 , Penrith NSW, 2751 , Australia
Telephone +61 2 4570 1125
Email hieinfo@lists.uws.edu.au
Description
URLs http://www.westernsydney.edu.au/hie

Summary of Files

Name Type Size Experiment Facility Download View
ENTOLAB_PK_CROSSFEC_R.csv RAW 5.2KB Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_VRGFEC_R.csv RAW 1.5KB Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_CORRPEM_R.csv RAW 775Bytes Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_CORRMD_R.csv RAW 1KB Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_TRANSEFF_R.csv RAW 458Bytes Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_VRGMATEDEGGS_R.csv RAW 1.6KB Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View
ENTOLAB_PK_QPCR_R.csv RAW 505Bytes Pezothrips kellyanus endosymbiotic associations Entomology Laboratory Growth Chamber Download View

Experiments

Pezothrips kellyanus endosymbiotic associations

ID Experiment_97
Parent Facility Facility - Entomology Laboratory Growth Chamber
Description Experiment testing the impact of the endosymbiotic bacteria Wolbachia and Cardinium on the reproductive outcomes of the thrips species Pezothrips kellyanus. Crossing experiments were performed using thrips that were uninfected, singly infected, or doubly infected with Wolbachia and Cardinium, and number of eggs and larvae were recorded.
Start date 2011-06-30
End date 2016-12-31
Subject cytoplasmic incompatibility, thrips, embryonic mortality, male development, Wolbachia, Cardinium, reproductive manipulation
Access Rights CC BY: Attribution (http://creativecommons.org/licenses/by/4.0)
FOR codes
Persistent URL http://w0297.uws.edu.au/org_level1/23/org_level2/97

Facilities

Entomology Laboratory Growth Chamber

Code EntoLab
Description This facility contains three temperature, light, and humidity controlled growth chambers, and is based in building L9 at the Hawkesbury Institute for the Environment, Western Sydney University.
Location -33.612, 150.749, ,
Parent Organisation Hawkesbury Institute for the Environment
Primary Contact gerard devine (g.devine@uws.edu.au)
Persistent URL http://w0297.uws.edu.au/org_level1/23

Files

ENTOLAB_PK_CROSSFEC_R.csv

Basic Information

Name ENTOLAB_PK_CROSSFEC_R.csv
Type RAW
Size 5.2KB
File format text/plain
Description Reproductive parameters were measured for crosses of haplodiploid Pezothrips kellyanus laboratory lines infected with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). The cross describes the infection status of the female parent x male parent. Reproductive parameters for the crossing combinations were measured over a ten day period; parameters are presented as number of eggs (Total_eggs, Hatched_eggs), percentage embryonic mortality (Embryonic_Mortality), number of Male, Female and total adult offspring (Total_Offspring), sex ratio as a percentage (Sex_Ratio) and percentage post embryonic mortality (Post_embryonic_mortality). Crossing combinations were selected to establish the effect of Cardinium, Wolbachia and co-infection on reproduction.
Labels Cardinium, cytoplasmic incompatibility, embryonic mortality, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2016-12-21 17:25:52 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/165657
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_VRGFEC_R.csv

Basic Information

Name ENTOLAB_PK_VRGFEC_R.csv
Type RAW
Size 1.5KB
File format text/plain
Description Reproductive parameters were measured for haplodiploid Pezothrips kellyanus laboratory lines infected with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). Reproductive parameters were collected for virgin female Pezothrips over a ten day period; fecundity presented as number of eggs (Total_eggs, Hatched_eggs, Unhatched_eggs), embryonic mortality as a percentage (Embryonic_Mortality), number of adult offspring (Male, Female) and sex ratio as a percentage (Sex_ratio).
Labels Cardinium, haplodiploid, Pezothrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2016-12-21 17:29:11 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/165658
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_CORRPEM_R.csv

Basic Information

Name ENTOLAB_PK_CORRPEM_R.csv
Type RAW
Size 775Bytes
File format text/plain
Description Levels of corrected post-embryonic mortality presented in incompatible crosses in Pezothrips kellyanus. Laboratory lines crossed were infected with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). The cross describes the infection status of the female parent x male parent. Crossing combinations were selected to establish the percentage post embryonic mortality (Post_embryonic_mortality).
Labels Cardinium, cytoplasmic incompatibility, embryonic mortality, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2017-01-06 14:56:28 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/167674
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_CORRMD_R.csv

Basic Information

Name ENTOLAB_PK_CORRMD_R.csv
Type RAW
Size 1KB
File format text/plain
Description Levels of male development presented in incompatible crosses in Pezothrips kellyanus. Laboratory lines crossed were infected with Cardinium (Ic), infected with both Cardinium and Wolbachia (Icw), or uninfected by either endosymbiotic bacteria (U). The cross describes the infection status of the female parent x male parent. Crossing combinations were selected to establish the percentage of male development (Male_Development).
Labels Cardinium, cytoplasmic incompatibility, male development, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2017-01-06 14:59:49 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/167675
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_TRANSEFF_R.csv

Basic Information

Name ENTOLAB_PK_TRANSEFF_R.csv
Type RAW
Size 458Bytes
File format text/plain
Description Maternal transmission efficiency of Cardinium and Wolbachia in Pezothrips kellyanus. The cross describes the infection status of the female parent x male parent, or the infection status of the virgin female parent.
Labels Cardinium, haplodiploid, maternal transmission, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2017-01-06 15:01:36 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/167676
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_VRGMATEDEGGS_R.csv

Basic Information

Name ENTOLAB_PK_VRGMATEDEGGS_R.csv
Type RAW
Size 1.6KB
File format text/plain
Description Egg number produced by virgin females and females mated with uninfected males over a period of ten days after mating. The cross describes the Cardinium and Wolbachia infection status of the female parent x male parent. This was to test whether bacterial infection and copulation influenced the fecundity, and to test the interaction of these two factors on the fecundity of Pezothrips kellyanus.
Labels Cardinium, fecundity, haplodiploid, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2017-01-06 15:05:48 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/167677
Parents
Children Pezothrips_endosymbiotic_coinfection.zip

ENTOLAB_PK_QPCR_R.csv

Basic Information

Name ENTOLAB_PK_QPCR_R.csv
Type RAW
Size 505Bytes
File format text/plain
Description Comparison of Cardinium and Wolbachia density in Pezothrips kellyanus females and males reared at 20°C. Quantitative PCR was used to measure the abundance of the single copy target genes gyrB (Cardinium), coxA and fbpA (Wolbachia) using gene-specific primers, and were normalised to the EF1a (host) reference gene to provide relative gene copy number per cell of the host thrips. Quantification cycle values (Cq) were normalised using the formula 2^-(dCq) where dCq is the difference between the Cq values of the reference gene and the target gene.
Labels Cardinium, qPCR, thrips, Wolbachia
Tags
Experiment Pezothrips kellyanus endosymbiotic associations
Facility Entomology Laboratory Growth Chamber
Date added 2017-01-06 15:07:46 +11:00
Added by Jennifer Morrow (j.morrow@westernsydney.edu.au)
Start date
End date
Persistent URL http://w0297.uws.edu.au/data_files/167678
Parents
Children Pezothrips_endosymbiotic_coinfection.zip